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| Registros recuperados: 396 | |
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| P. Kumar; C. M. Ramakritinan; A. K. Kumaraguru. |
| A bacterial strain, designated _Aeromonas sp. kumar_, was isolated from a water sample collected from Red tide Bloom occurred in the region of Gulf of Mannar region, Puthumadam Coast, India and the strain was identified using 16S rRNA based identification. During the sample collection, microbiology analysis was done to study the morphology of the bacteria. Pure culture of strain was maintained through out the study. DNA was isolated and sequenced using 16S rRNA primers. A length of 1452 nucleotide was sequenced and was put in public data base for obtaining accession number. The sequence was studied using MEGA 4, to estimate the evolutionary distances and to construct the Phylogenetic tree. Along with that Regulatory elements and Transcription factors were... |
| Tipo: Manuscript |
Palavras-chave: Ecology; Microbiology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3898/version/1 |
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| Xuri Wu; Nan Liu; Yunmian He; Yijun Chen. |
| Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Microbiology. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1697/version/1 |
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| Xuri Wu; Nan Liu; Yunmian He; Yijun Chen. |
| Various ketoreductases exclusively participate in all common biological events, and they are a class of important biocatalysts for the production of chiral alcohols. While many types of ketoreductase have been extensively studied and their functions, properties and utilities have been well known, the capability of stereoselectively reducing two carbonyl groups in the same diketohexanoate ester molecule to form a dihydroxy product by a single ketoreductase has not been evidently characterized. Here we show that a unique and novel enzyme, diketoreductase, was cloned from Acinetobacter baylyi, heterogeneously expressed in _Escherichia coli_ and purified to homogeneity. The diketoreductase is up to 78% homologous to bacterial 3-hydroxyacyl coenzyme-A... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Chemistry; Microbiology. |
| Ano: 2010 |
URL: http://precedings.nature.com/documents/1697/version/2 |
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| Satya N. V. Arjunan; Masaru Tomita. |
| Many important cellular processes are regulated by reaction-diffusion (RD) of molecules that takes place both in the cytoplasm and on the membrane. To model and analyze such multicompartmental processes, we developed a lattice-based Monte Carlo method, Spatiocyte that supports RD in volume and surface compartments at single molecule resolution. Stochasticity in RD and the excluded volume effect brought by intracellular molecular crowding, both of which can significantly affect RD and thus, cellular processes, are also supported. We verified the method by comparing simulation results of diffusion, irreversible and reversible reactions with the predicted analytical and best available numerical solutions. Moreover, to directly compare the localization... |
| Tipo: Manuscript |
Palavras-chave: Developmental Biology; Microbiology; Bioinformatics. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3845/version/1 |
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| Mahesh Satwekar; Abhijeet Satwekar. |
| Acute and chronic progression of injury to the kidney leads to the failure of the renal system and has become an increasingly important cause of morbidity and mortality. Present diagnosis detects the condition only after irreversible loss of 70 percent of kidney function. Current research is focused only on the clinical manifestations after the kidney injuries and not towards the exact cause of the condition. Here we propose a new outlook- that there is an involvement of a pathogen in the pathogenesis of kidney injuries. Basis for our proposal is given by the similarity of the pathogenesis events occurring between a classical example of hepatitis and kidney injuries. Furthermore, literature regarding the role of early kidney injury biomarkers in innate... |
| Tipo: Manuscript |
Palavras-chave: Biotechnology; Immunology; Microbiology. |
| Ano: 2012 |
URL: http://precedings.nature.com/documents/7021/version/1 |
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| Wriddhiman Ghosh; Prabir Haldar; Sabyasachi Bhattacharya; Jaideb Chatterjee; Prosenjit Pyne; Masrure Alam. |
| The theory envisaging the origin of plastids from endosymbiotic cyanobacteria is well-established but it is difficult to explain the evolution (spread) of plastids in phylogenetically diverse plant groups. It is widely believed that primordial endosymbiosis occurred in the last common ancestor of all algae^1^, which then diverged into the three primary photosynthetic eukaryotic lineages, viz. the Rhodophyta (red algae), Glaucocystophyta (cyanelle-containing algae) and Viridiplantae (green algae plus all land plants)^2^. Members of these three groups invariably have double membrane-bound plastids^3^, a property that endorses the primary endosymbiotic origin of the organelles. On the other hand, the three or four membrane-bound plastids of the evolutionary... |
| Tipo: Manuscript |
Palavras-chave: Microbiology; Evolutionary Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6607/version/1 |
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| Robert M. Sinclair. |
| Using a novel method, we show that ordered triplets of motifs usually associated with spliceosomal intron recognition are underrepresented in the protein coding sequence of complete Thermotogae, archaeal and bacterial genomes. The underrepresentation observed does not extend to the noncoding strand, suggesting that the cause of the asymmetry is related to mRNA rather than DNA. Our data do not suggest that the underrepresentation is due to gene transfer from eukaryotes. We speculate that one possible explanation for these observations is that the protein coding sequence of Thermotogae, Archaea and Bacteria was at some time in the past subjected to selection against certain motifs appearing in an order which might initiate splicing in environments harboring... |
| Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Microbiology; Bioinformatics; Evolutionary Biology. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/5770/version/1 |
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| Xiaoqing Su. |
| Mosquito causes problems in both disease transmission and disturbance to people's daily life. The controlling methods used now are mainly application of chemical pesticides which result in serious harm to environment. Biological control of the pest is one of the ideal ways. But, lack of suitable agent makes it difficult to extend this technique in wider scope. _Pythium guiyangense_ Su is a newly found fungal pathogen of mosquitoes and is promising for practical use. Here we summarize a list of mosquitoes that can be infected by the fungus so far, which shows the potential significance of the application of the fungus in the future. |
| Tipo: Manuscript |
Palavras-chave: Microbiology; Earth & Environment. |
| Ano: 2008 |
URL: http://precedings.nature.com/documents/1875/version/1 |
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| Spencer D. Heringa; Jonathan D. Monroe; James B. Herrick. |
| We are studying the lateral transfer of transmissible antibiotic resistance plasmids among stream bacteria impacted by fecal runoff from poultry and cattle. Such plasmids are typically large (ca. 40 – 100 kb) and occur in low copy numbers in the cell and have therefore typically been difficult to isolate and therefore to study. Traditional protocols, based upon variations of the standard alkaline-lysis method, are long (ca. 1 1/2 to 2 days) and difficult. Commercial kits designed for the isolation of Baterial Artificial Chromosomes (BACs) can be used and are an improvement; however, these are expensive and still require hours of sustained effort. We have adapted a method published by Rondon et al. (1999), originally designed for the isolation of... |
| Tipo: Poster |
Palavras-chave: Biotechnology; Genetics & Genomics; Microbiology. |
| Ano: 2007 |
URL: http://precedings.nature.com/documents/1249/version/1 |
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| Claudiu I. Bandea. |
| The cellular theory on the nature of life has been one of the first major advancements in biology. Viruses, however, are the most abundant life forms, and their exclusion from mainstream biology and the Tree of Life (TOL) is a major paradox in biology. This article presents a broad, unifying scenario on the origin and evolution of cellular and viral domains that challenges the conventional views about the history of life and supports a TOL that includes viruses. Co-evolution of viruses and their host cells has led to some of the most remarkable developments and transitions in the evolution of life, including the origin of non-coding DNA as a genomic protective device against viral insertion damage. However, one of the major fundamental evolutionary... |
| Tipo: Manuscript |
Palavras-chave: Genetics & Genomics; Immunology; Microbiology; Evolutionary Biology. |
| Ano: 2009 |
URL: http://precedings.nature.com/documents/3888/version/1 |
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| Elisabeth Jüschke; Bernd Marschner. |
| The reuse of reclaimed wastewater for irrigation of agricultural fields greatly influences the activity of soil microorganisms through the input of organic compounds. Due to the production of exoenzymes by microorganisms for the decomposition of substrates it can be assumed that the irrigation with treated wastewater (TWW) has a strong influence on the soil enzyme pool. In this study the activity of ten exoenzymes, which catalyses processes in C, N and P nutrient cycles, were determined in 3 different soils in 0-10, 10-20, 20-30, 30-50, 50-70 and 70-100 cm soil depth. The soils were used for agriculture and irrigated with reclaimed wastewater reused after a secondary treatment step. Additionally a control after freshwater irrigation was studied. Due to the... |
| Tipo: Presentation |
Palavras-chave: Ecology; Microbiology; Earth & Environment. |
| Ano: 2011 |
URL: http://precedings.nature.com/documents/6278/version/1 |
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| Silva,J.S.A.; Mota,R.A.; Pinheiro Júnior,J.W.; Almeida,M.C.S.; Silva,D.R.; Ferreira,D.R.A.; Azevedo,J.C.N.. |
| The objective of this study was to isolate and identify the aerobic bacterial microflora from the oral cavity mucosa and cloaca's samples, collected from Broad-snouted caiman (Caiman latirostris), born and bred in captivity at Parque Zoológico Arruda Câmara, João Pessoa, Paraíba, Brazil. The most common bacteria were Staphylococcus sp. (14.74%), Corynebacterium sp. (13.68%), Escherichia coli (13.68%) and Shigella sp. (11.58%), and the less common were Citrobacter sp. (1.05%), Klebsiella pneumoniae (1.05%) and Salmonella sp. (1.05%).This emphasizes the importance of these microorganisms' participation in infectious processes (sepsis) and injuries caused by crocodilians. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caiman latirostris; Cloaca; Crocodilians; Microbiology; Oral cavity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100034 |
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| Registros recuperados: 396 | |
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